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Figure 1: Formation and functionalization of subcellular compartments upon silk expression in E. coli.
 

In summary, our work provides a new compartmentalization strategy in the model prokaryotic organism E. coli, which not only demonstrates the feasibility of constructing artificial membraneless organelles within cells of prokaryotes, but also opens the door to broad applications in metabolic engineering and synthetic biology in the near future.

References

1. Qian, Z. G., Pan, F., Xia, X. X. Synthetic biology for protein-based materials. Curr.Opin. Biotechnol. 65, 197-204 (2020).

2. Xia, X. X. et al. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc. Natl. Acad. Sci. USA 107, 14059-14063 (2010).

3. Yang, Y. X., Qian, Z. G., Zhong, J. J., Xia, X. X. Hyper-production of large proteins of spider dragline silk MaSp2 by Escherichia coli via synthetic biology approach.Process Biochem. 51, 484-490 (2016).

4. Wei, S. P. et al. Formation and functionalization of membraneless compartments in Escherichia coli. Nat. Chem. Biol.(2020).     

https://www.nature.com/articles/s41589-020-0579-9.